Journal: Aging

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer.

doi: 10.18632/aging.205145

Figure Lengend Snippet: Figure 6. GO/KEGG analysis of gene SLC35A2 in colorectal carcinoma. (A) GO analysis of SLC35A2. The results were classified into Biological Process (BP), Cell Composition (CC) and Biological Function (MF). (B) KEGG analysis of SLC35A2 in colorectal tissues.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques:

Journal: Aging

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer.

doi: 10.18632/aging.205145

Figure Lengend Snippet: Figure 7. Construction of a nomogram to predict prognosis of patients with colorectal cancer. (A) Based on SLC35A2 expression and clinical characteristics of patients “TCGA-AA-A02K”, a nomogram was constructed. The 1, 3 and 5-years mortality rates of patient “TCGA-AA- A02K” were 0.292, 0.587 and 0.839, respectively. (B–D) The AUC of this nomogram with 1, 3 and 5-year ROC curves were 0.797, 0.8 and 0.818, respectively. (E–G) Calibration curves of 1, 3, and 5 years indicated that the nomogram was able to accurately predict colorectal cancer patients’ survival.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Construct