Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: The acquisition of the important gene SLC35A2 related to the prognosis. ( A , B ) When the partial likelihood deviation reaches a minimum, the optimal λ is determined and the LASSO coefficients of the most useful prognostic genes are further generated. ( C ) Survival analysis of TCGA database cohort. The results indicated that high expression of SLC35A2 was related to poor prognosis in colorectal cancer ( p = 0.014).

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Generated, Expressing

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: Analysis of pan-cancer SLC35A2 expression and survival analysis of methylation site cg26807389 related to SLC35A2 with CRC patients. ( A ) Differential expression of SLC35A2 was found in various tumor and normal tissues in TIMER database. Among them, the expression of SLC35A2 was elevated in colorectal adenocarcinoma (COAD) and rectal adenocarcinoma (READ). ( * p < 0.05, ** p < 0.01, *** p < 0.001). ( B ) Survival analysis of high expression group of methylation site cg26807389. The results showed that CRC patients with high expression of methylation site cg26807389 had poor prognosis ( p = 0.021).

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Methylation, Quantitative Proteomics

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: The relationship of SLC35A2 expression and clinical features in patients with colorectal cancer. ( A ) The expression of SLC35A2 in colorectal carcinoma was higher than that of normal tissues. ( B ) SLC35A2 expression was higher in Stages III and IV than in Stages I and II. ( C ) The difference of SLC35A2 expression between N0 and N1 was statistically significant ( p < 0.05). ( D – G ) The expression level of SLC35A2 in different sexes, age, M and T stages was not statistically significant ( p > 0.05). ( H ) The diagnostic value of SLC35A2 in CRC was assessed by drawing ROC and PRC curves. The AUC of the ROC curve is 0.834 and the PRC curve is 0.984 ( * p < 0.05, ** p < 0.01, *** p < 0.001, ns means p > 0.05). ( I ) Representative images of IHC staining of SLC35A2 in normal colon tissue (left) and colorectal cancer tissue (right) was presented.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Diagnostic Assay, Immunohistochemistry

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: Univariate analysis and multivariate analysis on overall survival in colorectal cancer patients.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques:

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: Analysis of immune microenvironment of colorectal cancer. ( A ) The overall immune cell infiltration of CRC patients. ( B ) The high-SLC35A2-expression and low-SLC35A2-expression groups differed in the level of immune cell infiltration, and only one distinct immune cell was obtained: plasma cells. ( C – F ) Correlation analysis indicated that there was significant correlation between 4 types of immunity and SLC35A2: macrophages M1 and M2, T cell regulatory (Tregs) and plasma cells. ( G ) One of the most significant immune cell types was obtained: plasma cells.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Clinical Proteomics

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: GO/KEGG analysis of gene SLC35A2 in colorectal carcinoma. ( A ) GO analysis of SLC35A2. The results were classified into Biological Process (BP), Cell Composition (CC) and Biological Function (MF). ( B ) KEGG analysis of SLC35A2 in colorectal tissues.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques:

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: Construction of a nomogram to predict prognosis of patients with colorectal cancer. ( A ) Based on SLC35A2 expression and clinical characteristics of patients “TCGA-AA-A02K”, a nomogram was constructed. The 1, 3 and 5-years mortality rates of patient “TCGA-AA-A02K” were 0.292, 0.587 and 0.839, respectively. ( B – D ) The AUC of this nomogram with 1, 3 and 5-year ROC curves were 0.797, 0.8 and 0.818, respectively. ( E – G ) Calibration curves of 1, 3, and 5 years indicated that the nomogram was able to accurately predict colorectal cancer patients’ survival.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Construct

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: Expression of SLC35A2 in colorectal cancer tissues and cells. ( A ) Representative immunohistochemical staining of SLC35A2 in CRC tumor tissues and matched paracancerous tissues. Strong positive expression of SLC35A2 expression in CRC tissues, weak positive of SLC35A2 expression in matched paracancerous tissues. Scale bar = 50 μm. ( B ) Western blotting was used to detect the protein expression of SLC35A2 in intestinal epithelium (NCM460) and colorectal carcinoma (HCT116, LOVO). ( C ) qRT-PCR was used to validate SLC35A2 expression in colorectal carcinoma. ( ** p < 0.01, *** p < 0.001).

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot, Quantitative RT-PCR

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

doi: 10.18632/aging.205145

Figure Lengend Snippet: Association between SLC35A2 expression and clinicopathological characteristics of colorectal cancer patients ( n = 60).

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Galectin-3 mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Galectin-3 protein expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by ELISA in chorionic villi tissue samples from patients in the SA and MA group. (A) The galectin-3 protein expression level is significantly higher in the MA group compared with the SA group. (B) The galectin-3 protein expression level in the early MA group (<4 weeks) is not significantly different compared with the SA group; whilst the galectin-3 protein expression level in the late MA group (>4 weeks) was significantly higher compared with the SA group. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; 4 w, 4 weeks.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Galectin-3 protein expression in SA and MA groups determined by IHC. Galectin-3 protein expression was examined in chorionic villi tissue samples from patients in the (A) negative control, (B) SA group and (C) MA group (magnification, ×400). Red arrows indicate galectin-3-positive expression in cells stained by DAB and blue arrows indicate cell nuclei counterstained with hematoxylin. SA, spontaneous abortion; MA, missed abortion; IHC, immunohistochemistry; DAB, diaminobenzidine.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Negative Control, Staining, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Quantification of galectin-3 protein expression in SA and MA groups determined by IHC. SA, spontaneous abortion; MA, missed abortion; IHC, immunohistochemistry.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Immunohistochemistry